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Pro-nuclear DNA micro-injection
is the oldest and most common method for mammalian transgenic creation (mainly transgenic mouse and rat). A solution of the transgene is injected into fertilized ovocytes
with a microsyringe under a microscope. These micro-injected ovocytes
are then transplanted in a surrogate mother. At birth the newborn are then tested for the transgene detection. After these tests,
the positive animals (founders) are intercrossed to obtain the F1
and F2 generation (transgenic mouse or transgenic rat line).
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recombination
